Open AccessComprehensive analysis of EGFR T790M detection by ddPCR and ARMS-PCR and the effect of mutant abundance on the efficacy of osimertinib in NSCLC patients
Author(s) -
Yue Li,
Yikai Xu,
Xiaoyan Wu,
Caiyun He,
Qing Liu,
Fang Wang
Publication year - 2019
Publication title -
journal of thoracic disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.682
H-Index - 60
eISSN - 2077-6624
pISSN - 2072-1439
DOI - 10.21037/jtd.2019.07.42
Subject(s) - osimertinib , t790m , digital polymerase chain reaction , medicine , lung cancer , mutation , oncology , microbiology and biotechnology , cancer research , epidermal growth factor receptor , cancer , polymerase chain reaction , biology , genetics , gene , erlotinib , gefitinib
Patients with non-small cell lung cancer (NSCLC) harboring epidermal growth factor receptor (EGFR) mutations often develop systemic disease progression during treatment with EGFR-tyrosine kinase inhibitors (TKIs). Droplet digital polymerase chain reaction (ddPCR) and amplification refractory mutation system (ARMS)-PCR are routinely applied for detection of EGFR mutations, including T790M, which is associated with TKI sensitivity. We compared the efficiency of ddPCR and ARMS-PCR in detecting T790M and explored the association between T790M abundance and osimertinib efficacy.