Open AccessMolecular identification of isolates from local microorganisms as potential biofertilizer
Author(s) -
Abdul Hasyim Sodiq,
Mieke Rochimi Setiawati,
Dwi Andreas Santosa,
Dedi Widayat
Publication year - 2021
Publication title -
sains tanah/sains tanah : jurnal ilmu tanah dan agroklimatologi
Language(s) - English
Resource type - Journals
eISSN - 2356-1424
pISSN - 1412-3606
DOI - 10.20961/stjssa.v18i2.44476
Subject(s) - bacillus cereus , biofertilizer , 16s ribosomal rna , microorganism , biology , microbial consortium , microbiology and biotechnology , food science , homology (biology) , bacteria , horticulture , biochemistry , gene , genetics
Local microorganisms (MOL) are liquid fertilizers commonly used by farmers to help increase crop production. Beneficial microbes in MOL need to characterize their interactions and ability to produce growth drive compounds. The purpose of this research is to identify the superior microbial isolates from MOL made by farmers from Cibodas Lembang Bandung, Indonesia that can produce phytohormones as biofertilizers. The results of the microbial selection of MOL derived from three best microbes are 1A-2 NFB, 4A-1 NFB, and 4B-1 NFB with the ability to produce auxin, i.e., 19.41 ppm, 17.18 ppm, and 10.59 ppm, respectively. The compatibility test between the three isolates showed negative results so that it was possible to apply three microbes as a consortium. The results of a molecular identification with a 16S rRNA analysis indicate strain microbe 1A-2 NFB: Bacillus cereus (99.88% homology), 4A-1 NFB: Bacillus cereus (99.76% homology), and 4B-1 NFB: Lysinibacillus sp. (99.88% homology).