Open AccessHubungan Genetik antara Domba Wonosobo (Dombos), Domba Ekor Tipis (DET) dan Domba Batur (Dombat) Melalui Analisis Polimorfisme Protein Darah
Author(s) -
F Noviani,
Sutopo Sutopo,
Edy Kurnianto
Publication year - 2017
Publication title -
sains peternakan/sains peternakan
Language(s) - English
Resource type - Journals
eISSN - 2548-9321
pISSN - 1693-8828
DOI - 10.20961/sainspet.v11i1.4798
Subject(s) - ceruloplasmin , transferrin , biology , locus (genetics) , albumin , breed , endocrinology , gene , genetics
The objective of this study was to evaluate the genetic relationships among Wonosobo sheep (Dombos), Thin-Tailed sheep (DET) and Batur sheep (Dombat) through blood protein polymorphism analysis by using electrophoresis Polyacrylamid Gel Electrophoresis-Thin Layer Electrophoresis (PAGE-TLE). The blood of the 20 head of Dombos (Wonosobo regency), 20 head of DET (Semarang regency) and 20 head of Dombat (Banjarnegara regency) were used as materials. Six locus of blood plasma protein were observed, then the gene frequency, individual heterosigosity, average heterosigosity and genetic distance among three breed were calculated. The results showed that the locus of pre-albumin (Pa), albumin (Alb), ceruloplasmin (Cp), transferrin (Tf), post-transferrin (P-tf) and amylase-I (Am-I), at DET, Dombos and Dombat were polymorphic (0,380-0,454). In conclusion, the DET and Dombat indicated the closer genetic relationship compared to DET-Dombos and Dombos-Dombat. Key words: sheep, polymorphism, genetic relationship