Isolasi dan Karakterisasi Fitase dari Mikroba yang Terdapat pada Pupuk Kompos, Rumen Sapi, Ragi dan Tanah Sawah

  This research was aimed to isolate and characterize  phytase from different source s of higger phosphat complex compound s (compost, cattle rumen, and yeast). Bacteria of phytase was isolated on Luria Bertani (LB) medium and it was incubated on 37 o C for 16 hours. Microbe from yeast was isolated on Pottato Dextrose Agar (PDA) medium. Crude enzyme from supernatant was extracted with centrifuge on 5.000 rpm for 5 minutes. Crude enzyme was characterized for optimum pH, temperature, and influence of matalo ion efector on relative activity of enzyme. B1 isolate from P88 compost had optimum relative activity on pH 5, temperature 50 o C and activator Zn 2+ (10 -3 and 10 -4 M). B2 Isolate from cattle rumen had optimum relative activity on pH 5, temperature 50 o C and activator Zn 2+ (10 -3 M) and Mg 2+ (10 -4 M). B3 isolated from soy sauce yeast had optimum relative activity on pH 5, temperature 60 o C, and  activators Mg 2+ (10 -3 M) and, Fe 2+ (10 -4 M). B4 isolated from tempeh yeast had optimum relative activity on pH 5, temperature 50 o C and activators Mg 2+ (10 -3 M) and Ca 2+ (10 -4 M). Key words : phytase, optimum pH, optimum temperature, metalo ion efector, isolate.