Open AccessKeragaman genotipik entok (Cairina moschata) berdasarkan polimorfisme protein darah
Author(s) -
Sutopo Sutopo,
Diah Ayu Lestari,
Asep Setiaji,
Edy Kurnianto
Publication year - 2021
Publication title -
livestock and animal research
Language(s) - English
Resource type - Journals
eISSN - 2721-7086
pISSN - 2721-5326
DOI - 10.20961/lar.v19i3.54921
Subject(s) - microbiology and biotechnology , biology , chemistry
Objective: The aim of this study was to identified genetic diversity of muscovy duck in Central Java through blood protein polymorphisms by using electrophoresis method. Methods: Blood sample was collected from a total of 60 muscovy ducks from the districts of Demak, Magelang and Pekalongan, 20 samples each. The Electrophoresis Polyacrylamide Gel of Electrophoresis–Thin Layer Electrophoresis (PAGE-TLE) was used in this study. Parameters observed were protein of albumin (Alb), ceruloplasmin (Cp), transferrin (Tf) and amylase-I (Am-I). Gene frequency, heterozygosity (Ho) and genetic distance were analyzed by using DISPAN program.Results : The results showed that the Alb, Cp, Tf and Am-I of muscovy duck showing polymorphic characters. Gene frequency of Alb B (0.61) was higher compared to Alb A (0.39). Gene frequency of Cp A (0.62) was higher than the Cp B (0.38). Furthermore, gene frequency of Tf A and Tf B were 0.32 and 0.68, respectively as well as gene frequency of Am-I A (0.82) was higher than Am-I B (0.18). Muscovy duck population of Demak showed higher Ho value for Alb and Am-I, whereas Magelang showed higher value for Cp and Tf. Population of muscovy duck of Demak showed closer genetic distance to Pekalongan than Magelang. Conclusions: The protein blood locus of three population of muscovy duck in Central Jawa showed polymorphic characters.