Open AccessPreparation of Silver-Chitosan Nanocomposites Colloidal and Film as Antibacteri Material
Author(s) -
Endang Susilowati,
Mohammad Masykuri,
Maria Ulfa,
Dyah Ayu Puspitasari
Publication year - 2020
Publication title -
jkpk (jurnal kimia dan pendidikan kimia)
Language(s) - English
Resource type - Journals
eISSN - 2503-4154
pISSN - 2503-4146
DOI - 10.20961/jkpk.v5i3.46711
Subject(s) - chitosan , nanocomposite , silver nanoparticle , materials science , nuclear chemistry , surface plasmon resonance , colloid , scanning electron microscope , absorption (acoustics) , chemical engineering , nanoparticle , silver nitrate , nanotechnology , chemistry , composite material , engineering
Colloidal nanocomposites silver-chitosan have been made. Silver nanoparticles were produced by chemical reduction methods assisted microwave irradiation using chitosan from crab shells as a reducing agent and stabilizer, AgNO 3 as a precursor and NaOH as an accelerator. This study investigated AgNO 3 concentration toward localized surface plasmon resonance (LSPR) phenomenon of nanocomposites colloidal. The size and shape of the silver nanoparticles were confirmed by TEM. Furthermore, the stability of the storage was observed for twelve weeks. Colloidal and film nanocomposites silver- chitosan have been made by casting method by drying at room temperature. After that, the film characterization was carried out, including swelling with gravimetry methods and surface morphology using scanning electron microscopy (SEM). Diffusion methods tested colloid antibacterial activity and silver-chitosan nanocomposite film’s against E. Coli and S. Aureus . The results showed that the formation of silver nanoparticles was identified by the LSPR absorption band's appearance at 413-419 nm. The increasing of AgNO 3 concentration increased the intensity of the LSPR absorption band. Silver nanoparticles with sizes of about 3-9 nm are spherical. The silver nanoparticles were stable at 12 weeks of storage. The higher AgNO 3 concentration tends to increase the swelling of the film. The surface of the silver-chitosan nanocomposite film’s was rougher than that of the chitosan film. The higher the silver nanoparticle concentration, the higher the colloid and film antibacterial activity against E. Coli and S. Aureus.