Open AccessRelation of serum IL-32 levels and gene polymorphism rs45499297 with obesity in Mexican patients: a laboratory and in silico analysis
Author(s) -
Luz Andrea Martínez Pérez,
Julieta Saraí Becerra-Ruiz,
Juana Esther García-Aviña,
Grecia Denisse González-Sánchez,
Fernando Martínez-Esquivias,
Sonia Isela Vázquez-Jiménez,
Saúl Ramírez-De Los Santos,
Edgar Iván López-Pulido,
Juan Manuel Guzmán-Flores
Publication year - 2022
Publication title -
nutrición hospitalaria
Language(s) - English
Resource type - Journals
eISSN - 1699-5198
pISSN - 0212-1611
DOI - 10.20960/nh.03804
Subject(s) - genotype , in silico , underweight , overweight , biology , obesity , promoter , single nucleotide polymorphism , gene , medicine , allele , endocrinology , genetics , gene expression
Background: many genes have been involved in the development of obesity. Interleukin 32 (IL-32) is a proinflammatory cytokine; rs45499297 is a T/C promoter, single-nucleotide polymorphism of the IL32 gene. Objectives: this study aimed to evaluate the rs45499297 polymorphism and its association with obesity. Another objective of this study was to carry out an in silico analysis. Methods: this study was cross-sectional, and included 333 subjects classified by body mass index and fat percentage. The plasma glucose and lipid profile were measured. We measured serum IL-32 protein by ELISA and the rs45499297 polymorphism by PCR-RFLP. We used several databases to build the IL32 gene network and infer transcription factors that bind to this polymorphic site. Results: subjects underweight and with low fat percentages had lower levels of IL-32. CT genotype and allele C were less frequent in the overweight/obesity group than in the normal-weight group. Interestingly, this result remained only in the male gender. We found that the transcription factors Hepatocyte Nuclear Factor and Specificity Protein 1 bind to this polymorphic site. In addition, we infer that IL32 is involved in metabolic pathways related to viral infections. Conclusion: the TC genotype is associated with overweight/obesity. The decrease in levels of IL-32 observed in underweight and low fat percentage groups could be due to an impaired inflammatory profile. The in silico analysis showed that several transcriptional factors bind at this polymorphic site, and that the enrichment of the metabolic pathways is diverse.