Open AccessSimple Thin-Layer Chromatographic and UV-Spectrophotometric Analysis of Promethazine and its N-Demethylation Metabolites from Biological Fluids
Author(s) -
Samuel J. Bunu,
Diepreye Ere,
Wilson O. Diana
Publication year - 2020
Publication title -
international journal of pharmtech research
Language(s) - English
Resource type - Journals
eISSN - 2455-9563
pISSN - 0974-4304
DOI - 10.20902/ijptr.2019.130402
Subject(s) - chromatography , cyp2d6 , promethazine , thin layer chromatography , chemistry , demethylation , population , drug metabolism , cytochrome p450 , pharmacology , metabolism , biology , medicine , biochemistry , environmental health , gene expression , gene , dna methylation
Several Ultra-Violet spectrophotometric analytical method has been made availablein the analysis of phenothiazine group of drugs but Thin-Layer Chromatographic techniques arenot fully utilized. Hence, we were able to develop a succinct, simple and cost-effective TLCand UV-spectrophotometric quantification method for the analysis promethazine andmetabolites from biological fluids, validating previous studies. The proposed method was foundto be precise, accurate and phenotypic determination and categorization were successfullyestimated among the test samples. From the chromatogram intensities, 33.3% of the studypopulation were classified as poor metabolizers, 40% were intermediate metabolizers, and26.67% extensive metabolizers. Little or no elimination of N-desmethylpromethazine wasobserved for subjects with poor metabolism in correlation with the severity. Therefore, thephenotypic knowledge will help in the clinical choices as an individual from the same familymay likely metabolize a giving drug in the same manner due to their genetic similarities.Keywords : Promethazine, Metabolites, Thin-Layer Chromatography, cytochrome P450.