Open AccessA New Stability Indicating RP-HPLC Method for Determination of Chlorthalidone, Telmisartan and Cilnidipine in Bulk and Tablet Dosage Form
Author(s) -
Shahnaz Sultana,
Salomi Patta,
T. Vimalakkannan,
K. Ravindra Reddy
Publication year - 2020
Publication title -
international journal of pharmtech research
Language(s) - English
Resource type - Journals
eISSN - 2455-9563
pISSN - 0974-4304
DOI - 10.20902/ijptr.2019.130106
Subject(s) - telmisartan , chlorthalidone , chemistry , chromatography , potassium , pharmacology , diuretic , medicine , endocrinology , organic chemistry , blood pressure
In present study, accurate, precise, rapid and sensitive stability indicting HPLC-UVmethod has been established for quantification of Telmisartan, Cilnidipine and Chlorthalidonesimultaneously in Tablet and bulk. Telmisartan, Cilnidipine and Chlorthalidone were resolutedon Sunsil C18 column (4.6mmx250mm; 5μm) using mobile phase containing Acetonitrile andPotassium dihydrogen phosphate in 50:50(v/v) ratio with flow rate of 1ml/min at 238 nm.Concentrations were linear over the range of 40-120 μg/ml for Telmisartan, 10-30 μg/ml forCilnidipine and 6.25-18.75 μg/ml for Chlorthalidone. The percentage recovery was found to be99.70-100.51% for Telmisartan, 98.41-100.49% for Cilnidipine and 99.34-100.48% forChlorthalidone. % RSD for peak area was 0.069% for Telmisartan, 0.058% for Cilnidipine and0.057% for Chlorthalidone shows that the proposed method is precise. Force-degradationstudies have not shown any observable change in the results and hence the proposed method isstability indicating and hence the method is suitable for routine analysis of Telmisartan,Cilnidipine and Chlorthalidone in bulk and tablet dosage form.