Open AccessDevelopment & Validation of HPLC method for simultaneous estimation of Drotaverine & Omeprazole
Author(s) -
Zaheer Shaikh,
Abdul Ahad,
Showkat Patel,
Maqdoom Farooqui
Publication year - 2020
Publication title -
international journal of chemtech research
Language(s) - English
Resource type - Journals
eISSN - 2455-9555
pISSN - 0974-4290
DOI - 10.20902/ijctr.2019.130101
Subject(s) - chromatography , omeprazole , chemistry , high performance liquid chromatography , potassium , acetonitrile , potassium phosphate , selectivity , pharmacology , catalysis , biochemistry , organic chemistry , medicine
A simple and accurate method was developed for simultaneous estimation ofDrotaverine & Omeprazole. The chromatography parameters includes stainless steel column Develosil ODS HG-5 RP C18, 5m, 15cmx4.6mm i.d and acetonitrile: potassium dihydrogen phosphate buffer (0.02M, pH 3.0) (55:45v/v) as mobile phase at a flow rate (1.0 ml/minute). The determinations were performed using UV-Vis detector set at 273 nm. The developed HPLC method showed specificity and selectivity with good precision and accuracy, which makes it very suitable for quantification of Drotaverine & Omeprazole.