Open AccessCOMPARISON OF ANTIOXIDANT ACTIVITIES OF TESPONG HERBAL (OENANTHE JAVANICA DC.) EXTRACT AND NANOEXTRACT USING DPPH METHOD
Author(s) -
In Rahmi Fatria Fajar,
Dewi Rahma Fitri,
Nisa Fitriyani,
Michael Chuanvin
Publication year - 2021
Publication title -
jurnal ilmiah farmasi
Language(s) - English
Resource type - Journals
ISSN - 1693-8666
DOI - 10.20885/jif.vol17.iss1.art1
Subject(s) - maceration (sewage) , traditional medicine , dpph , antioxidant , bioavailability , chemistry , herb , extraction (chemistry) , zeta potential , polyphenol , herbal extracts , food science , chromatography , medicine , medicinal herbs , pharmacology , nanoparticle , biochemistry , nanotechnology , materials science , composite material
Background: Tespong herbs (O. Javanica DC.) contain numerous chemical compounds considered beneficial for medications, including flavonoids, phenols, and tannins, which can function as antioxidants. Nanoparticles are materials with a size range of 1-1000 nanometers which can increase the bioavailability of drugs, thus accelerating the drug delivery system in the body. Objective: The aim of this study is to identify the characteristics of herbal extract and nanoextract of Tespong and to compare their antioxidant activity. Methods: Extraction of tespong herbs was done using maceration for three days, nanoextract was formulated using the ionic gelation method with herbal extract of tespong, chitosan, and Na-TPP. Antioxidant activity testing was carried out using the DPPH method. Results: The water content of tespong herbal extract was 1.78%, and the total ash content was 4.95%. The tespong herbal nanoextract was 148.1nm in diameter with a polydispersity index of 0.362 and a zeta potential value of -39.16 mV. The IC50 values of tespong herbal extract and nanoextract were 65.424 ppm and 97.106 ppm, respectively, which fell into the strong category. Conclusion: Tespong herbal extract had a higher antioxidant activity compared to its nanoextract. Keywords: Tespong herb, nanoextract, chitosan, Na-TPP