Open AccessRecovery from bone loss, diminished mineral density and strength in mice after treatment with steroidal and nonsteroidal anti-inflammatory drugs by injection of exosomes enriched with agomir miRNAs
Author(s) -
Tomasz P. Lehmann,
Ewa PruszyńskaOszmałek,
Paweł Kołodziejski,
Magdalena Wojtków,
Celina Pezowicz,
Mirosław Szybowicz,
Paweł P. Jagodzińśki,
Marek Nowicki,
A. Trzaskowska,
S. Mielcarek,
Maciej Głowacki
Publication year - 2019
Publication title -
journal of medical science
Language(s) - English
Resource type - Journals
eISSN - 2353-9801
pISSN - 2353-9798
DOI - 10.20883/medical.379
Subject(s) - methylprednisolone , diclofenac , bone mineral , pharmacology , bone remodeling , osteoporosis , inflammation , anti inflammatory , nonsteroidal , microrna , medicine , prostaglandin e2 , endocrinology , chemistry , biochemistry , gene
The project “Recovery from bone loss, diminished mineral density and strength in mice after treatment with steroidal and nonsteroidal anti-inflammatory drugs by injection of exosomes enriched with agomir miRNAs” is an animal experiment project, and an attempt to apply small RNA strands to reverse the harmful effects of anti-inflammatory drugs. Patients chronically treated with an anti-inflammatory drug suffer from musculoskeletal side effects, including reversed mineralisation and disabled bone fracture healing. The aims of the study are to measure changes in bone mineral density and bone strength in mice treated with methylprednisolone or diclofenac in combination with treadmill exercise. The reversal of the negative effects of these drugs will be assayed using modified miRNA agomir. Bones obtained from the treated mice will be analysed using micro-CT, dynamic mechanical analysis (DMA), nanoindentation, Raman spectroscopy and gene expression. We expect to find specific miRNA counteracting the demineralisation of the mice bones caused by methylprednisolone or diclofenac.