Open AccessSeleção de cepas de Bacillus thuringensis Berliner para o controle de Aedes aegypti Linnaeus
Author(s) -
André Luiz de Almeida Melo,
André Luís Lopes da Silva,
Magda Clara Vieira da Costa Ribeiro,
Mitiyo Fukuda Miyaoka,
Vanete Thomas Soccol,
Carlos Ricardo Soccol
Publication year - 2013
Publication title -
journal of biotechnology and biodiversity
Language(s) - English
Resource type - Journals
ISSN - 2179-4804
DOI - 10.20873/jbb.uft.cemaf.v4n1.melo
Subject(s) - bacillus thuringiensis , aedes aegypti , biology , microbiology and biotechnology , bacteria , bacillales , strain (injury) , larva , gel electrophoresis , botany , bacillus subtilis , genetics , anatomy
The present study aimed to select strains of Bacillus thuringiensis with insecticidal activity against Aedes aegypti. It was tested sixteen strains of bacteria, isolated from Paraná state, Brazil, that were used in laboratory assays with mosquito larvae. Tests were carried out in two stages, first one to select the most efficient strains (screening) and second to estimate LC50. The protein profile of the highest toxicity of strain was obtained by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). The best performance of larval mortality was produced by BR-01 strain, which 96.7% mortality rate, significantly higher than others. In the second part, there was obtained a LC50 of 9.07 µL.L-1 fermented extract. The protein profile revealed many peptides between 15 and 140kDa, similar to that reported in Bacillus thuringiensis ser. israelensis strains which high toxicity to mosquito species.