Open Access<em>In vitro</em> neoformation of grape chimeras (<em>Vitis vinifera</em> L.)
Author(s) -
Sandrine Verdisson,
Fabienne Baillieul,
JeanClaude Audran
Publication year - 1999
Publication title -
oeno one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.516
H-Index - 13
ISSN - 2494-1271
DOI - 10.20870/oeno-one.1999.33.1.1042
Subject(s) - cultivar , biology , horticulture , botrytis cinerea , shoot , botany
Difference in grape sensitivity to Botrytis cinerea attacks between cultivars was explained by differences in the epidermic tissue of the fruit. Therefore, this work was conducted to create a grape periclinal chimera, whose fruits would combine the skin of a Botrytis tolerant cultivar with a pulp of an another cultivar admitted its good organoleptic quality and productivity. In a first time, graftings of two cultivars (Chardonnay and Pinot noir) were conducted in vitro on 5 different media supplemented with various plant growth regulators. Adventitious shoots were only observed on medium containing BAP and GA3 from a mixed callus structure after four weeks of darkness followed by a light/dark regime. In a second time, RAPD analysis, conducted on these plants, showed their chimerical characteristics.