Open AccessTwo-dimensional and three-dimensional cell culture models in vitro: pros and cons
Author(s) -
Е. С. Галимова,
М. М. Галагудза
Publication year - 2018
Publication title -
bûlletenʹ sibirskoj mediciny
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.135
H-Index - 3
eISSN - 1819-3684
pISSN - 1682-0363
DOI - 10.20538/1682-0363-2018-3-188-196
Subject(s) - computational biology , in vitro , relevance (law) , cancer , cons , cancer cell lines , 3d cell culture , cell culture , cancer cell , biology , key (lock) , computer science , genetics , political science , ecology , law , programming language
Discovery and development of new chemical compounds with putative anti-cancer properties requires reliable predictive preclinical models for in vitro screening of efficacy. Such models mainly include cultures of human cancer cells: two-dimensional (2D) and three-dimensional (3D) cell culture systems. In this review, we discuss the molecular aspects of cells cultured in 2D and 3D, and their relevance to cancer study, focusing on key examples from the recent literature. Advantages, disadvantages and perspectives of described models are also analyzed.