Open AccessDEVELOPMENT OF OPTIMAL EXPERIMENTAL THERAPEUTIC REGIMENS ADMINISTRATION OF IMMOBILIZED INTERFERON α-2b
Author(s) -
О. Г. Шитикова,
Е. Ю. Шерстобоев,
H. В. Масная,
М. Г. Данилец,
Е. С. Трофимова,
А. А. Лигачева,
П. Г. Мадонов,
Д. Н. Киншт,
Константин Ершов,
М. А. Шилова
Publication year - 2014
Publication title -
bûlletenʹ sibirskoj mediciny
Language(s) - English
Resource type - Journals
eISSN - 1819-3684
pISSN - 1682-0363
DOI - 10.20538/1682-0363-2014-5-114-121
Subject(s) - immune system , splenocyte , phagocytosis , drug , in vivo , pharmacology , immunology , therapeutic effect , interferon , medicine , antibody , biology , microbiology and biotechnology
The development of optimal experimental therapeutic schemes of immobilized interferon α-2b (production of JSC «Siberian Center for Pharmacology and Biotechnology», Novosibirsk) administration was conducted in the frame of the common program of pre-clinical study. The drug being studied was administered intragastrically for 3, 5 and 7 days in the therapeutic dose (1.8 × 10 5 IU/kg). Experimental evaluation of immunotropic properties of immobilized interferon α-2b was carried out at in vivo experiments in outbred CD1 male mice with using of following tests: the study of drug effect on the phagocytic activity of peritoneal leukocytes (the relative number of phagocytes and the number of bacteria which were assimilated by phagocytes were assessed); the study of drug effect on the humoral immune response (the total number of splenocytes, the relative and absolute number of antibody-forming cells and their functional activity were determined); estimation of drug effect on the cellular immune response with the using of delayed-type hypersensitivity test induced by sheep red blood cells. Based on these data we concluded that course administration of immobilized interferon α-2b in the therapeutic dose for 5 days increased phagocytosis of neutrophils and humoral immune response most effectively. Cell-mediated immune response was significantly increased after 7-fold administration of studied drug.