Open AccessEffect of alloxan on glutathione system and oxidative protein modification in adipocytes of rats at experimental diabetes
Author(s) -
В. В. Иванов,
Е. В. Шахристова,
Е. А. Степовая,
В. В. Новицкий
Publication year - 2011
Publication title -
bûlletenʹ sibirskoj mediciny
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.135
H-Index - 3
eISSN - 1819-3684
pISSN - 1682-0363
DOI - 10.20538/1682-0363-2011-3-44-47
Subject(s) - glutathione , oxidative stress , adipose tissue , chemistry , antioxidant , thiobarbituric acid , glutathione reductase , alloxan , medicine , oxidative phosphorylation , endocrinology , glutathione disulfide , lipid peroxidation , biochemistry , diabetes mellitus , biology , enzyme , glutathione peroxidase
There was carried out a research of the development of oxidative stress, the condition of glutathione dependant system of antioxidant protection in adipocytes of epididymal adipose tissue of rats when injecting alloxan. The development of oxidative stress in adipocytes was characterized by the increase of lipids hydroperoxide concentration, products reacting with thiobarbituric acid, and the increase of carbonylderived protein. Redox-condition in adipocytes was considerably changing that was specified by the decrease of the content of the reduced form of glutathione and tendency to the increase of glutathione disulfide content, decrease of ratio between reduced and oxidized forms of threepeptide. Damage of protein molecule at oxidative stress may lead to the abnormality of transduction of insulinic signal and appearance of insulin resistance in adipose tissue.