Open AccessRole of protein kinases JNK and p38 in regulation of mononuclear leucocytes apoptosis in oxidative stress
Author(s) -
N. Yu. Chasovskikh
Publication year - 2008
Publication title -
bûlletenʹ sibirskoj mediciny
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.135
H-Index - 3
eISSN - 1819-3684
pISSN - 1682-0363
DOI - 10.20538/1682-0363-2008-3-38-43
Subject(s) - oxidative stress , peripheral blood mononuclear cell , p38 mitogen activated protein kinases , kinase , apoptosis , annexin , reactive oxygen species , microbiology and biotechnology , hydrogen peroxide , chemistry , oxidative phosphorylation , programmed cell death , intracellular , in vitro , immunology , biology , biochemistry , protein kinase a
Programmed cell death of peripheral blood mononuclear leucocytes taken from healthy donors and cultivated with various concentration of Н 2 О 2 , selective inhibitors of JNK (SP600125), 38 (ML3403) and in case of pneumonia was investigated. Intensification of intracellular production of reactive oxy р МАРК - gen species was accompanied by the increase in number of apoptotic and TNFR1-presented cells and mononuclears with reduced value of mitochondrial transmembrane potential in a case of oxidative stress induction with 1 mM hydrogen peroxide and in blood taken from patients with pneumonia. Action of inhibitors SP600125 and ML3403 in vitro in oxidative stress conditions prevents the increase in number of annexin- positive mononuclear cells, that confirms the participation of JNK and 38 -kinases in mechanisms of oxidative stress-mediated apoptosis dysregulation.