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ISOLASI DAN UJI SITOTOKSIK SENYAWA LIMONEN DARI KULIT BATANG KASTURI (Mangifera casturi)
Author(s) -
Kholifatu Rosyidah
Publication year - 2018
Publication title -
bioscientiae
Language(s) - English
Resource type - Journals
eISSN - 2808-4438
pISSN - 2808-1838
DOI - 10.20527/b.v8i2.191
Subject(s) - chemistry , chromatography , parthenolide , gas chromatography , traditional medicine , medicine , biochemistry , apoptosis
The air-stem barks of M.casturi were extracted with Methylene Chloride (MTC). The MTC extract’s had treatment with Brine Shrimp Lethality Test (BSLT) than analyzed with probit and resulted LC50= 47,31 ppm. MTC extract’s was fractionated by Vacuum Liquid Chromatography and recrystalization obtained pure compounds. The compounds was analyzed with GC-MS (Gas chromatography-Mass Spectroscopy) and cytotoxic test with cancer cell Leukemia P388. This compound is limonene and have IC50=70 ppm.

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