Open AccessAKTIVITAS ANTIBAKTERI FRAKSI SAPONIN DARI KULIT BATANG TUMBUHAN KASTURI (Mangifera casturi)
Author(s) -
Kholifatu Rosyidah,
Syana Asri Nurmuhaimina,
Noer Komari,
Maria Dewi Astuti
Publication year - 2018
Publication title -
bioscientiae
Language(s) - English
Resource type - Journals
eISSN - 2808-4438
pISSN - 2808-1838
DOI - 10.20527/b.v7i2.181
Subject(s) - saponin , mangifera , chemistry , antibacterial activity , bark (sound) , silica gel , chromatography , fraction (chemistry) , traditional medicine , column chromatography , methanol , bacteria , botany , biology , organic chemistry , medicine , alternative medicine , pathology , ecology , genetics
This research to study antibacterial activity (E.coli and S.aureus) of saponin fractions from stem bark of Mangifera casturi. Powdered of stem bark of M.casturi (512,67 g) were extracted with methanol. Methanol extract (32.57 g) was partitionated with n-hexane to give saponin extract. Saponin extract (5.98 g) was partitionated by Vacuum Liquid Chromatography (VLC) over silica gel to give 39 fractions were grouped into four major fractions A-D by combining fractions with similar TLC profile. Every fractions studied antibacterial activity with E. coli and S. Aureus with difusi methode. Fraction A most active then other fraction with inhibition diameter 10.3 ± 0.5 mm (E.coli) and 10.8 ± 0.3 mm (S.aureus).