Open AccessStandardization of an Efficient Protocol for In Vitro Micropropagation of Centella asiatica L. – An Important Medicinal Plant
Author(s) -
Sammera Siddiqui,
Tessy Thomas,
Shagufta khan
Publication year - 2019
Publication title -
pharmaceutical and biosciences journal
Language(s) - English
Resource type - Journals
ISSN - 2582-0540
DOI - 10.20510/ukjpb/7/i4/185561
Subject(s) - centella , shoot , micropropagation , explant culture , acclimatization , auxin , murashige and skoog medium , biology , axillary bud , meristem , botany , horticulture , in vitro , biochemistry , gene
The present study was undertaken to evaluate the most suitable and efficient media of low concentration growth regulators for shoot induction and subsequent multiplication in Centella asiatica L. an important and valuable medicinal plant. The best shoot induction (82±2.2%) was found in MS medium supplemented with 0.5 mg/ l BAP + 0.1 mg/ l NAA from axillary meristem explant. Combination of 0.5 mg/ l BAP + 0.5 mg/ l NAA and 0.5 mg/ l BAP + 0.5 mg/ l KN gave the most effective shoot multiplication from initiated meristem cultures. The elongated shoots were rooted in MS medium supplemented with different concentrations of auxins (NAA and IBA). Lower concentration of IBA was more suitable for root induction when compared to NAA. The success of plant tissue culture for in vitro culture of Centella asiatica was encouraged by acclimatization of the plantlets in the field conditions. About 95% plantlets survived under field conditions.