Open AccessGenetic Stability The Protein Encoding Envelope (E) Genes Dengue Virus Serotype-4 Passaged in Vero Cell As A Candidate Chimera Vaccine Material
Author(s) -
Deya Karsari
Publication year - 2020
Publication title -
journal of stem cell research and tissue engineering
Language(s) - English
Resource type - Journals
eISSN - 2614-1264
pISSN - 2614-1256
DOI - 10.20473/jscrte.v4i2.22748
Subject(s) - vero cell , virology , dengue virus , biology , gene , dengue fever , virus , serotype , dengue vaccine , nucleotide , dna , amino acid , microbiology and biotechnology , genetics
This study aims to analyze genetic stability of the gene encoding the envelope protein (E) dengue virus serotype-4 passaged in vero cells, Denv-4 passaged in vero cells serially then continued with RNA extraction at passage 0, 10, 20, 30, 40 ,50 , and 60, and then continued with two step PCR and amplification, and sequencing then analyze the nucleotide stability with BLAST and MEGA 5 software. The result shows that there are many variable site in nucleotide and amino acid with high mutation rate 57.4% for nucleotide and 71.9% for amino acid ,while the similarity between passages are high ranging from 91% - 98%. The conclusion for this study is Denv-4 after analyzed shows that the gene encoding protein E has many variable site but high in similarity.