Open AccessEffect of Vaccination Outer Membrane Protein 52 kDa on Changes in Erythrocyte Index of Tilapia (Oreochromis niloticus) Infected by Aeromonas hydrophila
Author(s) -
Nanda Rino Nurrahmad,
M. Gandul Atik Yuliani,
Rahaju Ernawati,
Sri Chusniati,
Eduardus Bimo Aksono Herupradoto,
Retno Sri Wahyuni
Publication year - 2021
Publication title -
journal of basic medical veterinary
Language(s) - English
Resource type - Journals
ISSN - 2774-9959
DOI - 10.20473/jbmv.v10i1.28592
Subject(s) - oreochromis , aeromonas hydrophila , tilapia , biology , aeromonas , microbiology and biotechnology , vaccination , veterinary medicine , nile tilapia , hematology , red blood cell , immunology , fish <actinopterygii> , bacteria , medicine , fishery , genetics
The purpose of this study was to determine the effect after being vaccinated by OMP 52 kDa Aeromonas hydrophila for 1 week and then infected with Aeromonas hydrophila 10 CFU/mL for 4 days on changes in erythrocyte index. Tilapia (Oreochromis niloticus) used in this study was 10-12 cm long. There were 20 tilapia (Oreochromis niloticus) which were divided into 4 groups, consisting of 2 control groups and 2 treatment groups which were given various types of vaccine formulations. Group P0 (-) (unvaccinated and infected), Group P0 (+) (unvaccinated and infected), group P1 (vaccinated with the whole cell protein "HydroVac®" and infected), and P2 (vaccinated with Outer Membrane Protein 52 kDa and infected) by intramuscular injection. Post-treatment blood samples were collected on day 5 post-infection, collected through a caudal punctie and then analyzed using a hematology analyzer. Post-treatment outcomes led to statistically significant changes (p < 0.05). Therefore, the vaccine caused a significant change in the erythrocyte index.