Open AccessMMUNOHISTOCHEMICAL ANALYSIS OF NF-kB (P0/P) IN PATIENT WITH AGGRESSIVE AND CHRONIC PERIODONTITIS
Author(s) -
Chiquita Prahasanti
Publication year - 2013
Publication title -
indonesian journal of tropical and infectious disease/indonesian journal of tropical an infectious disease
Language(s) - English
Resource type - Journals
eISSN - 2356-0991
pISSN - 2085-1103
DOI - 10.20473/ijtid.v4i4.235
Subject(s) - chronic periodontitis , periodontitis , aggressive periodontitis , p50 , medicine , immunohistochemistry , inflammation , dentistry , transcription factor , biology , gene , genetics
Background: Nuclear factor-kappaB (NF-κB) is a protein complex that plays a role in transcription factors and in response to inflammation. Periodontitis is a periodontal disorder caused by various bacteria such as A. actinomycetemcomitan and P.gingivalis whose LPS is closely related to NFκB (p50/p65). Aim: This study observed whether NF-κB (p50/p65) played a role in aggressive and chronic periodontitis. Methods: Data were obtained from periodontal tissue 40 patients with aggressive periodontitis and 40 patients with chronic periodontitis. Samples were derived from periodontal tissue with abnormalities and NFκB (p50/p65) protein expression test was performed by immunohistochemistry. The statistical test used was the t-test. Results: In NF-κB (p50) the t value was -12 041 and significance 0.000, with α = 5%, showing significant difference in protein expression of NF-κB (p50) between patients with aggressive periodontitis and chronic periodontitis. OR estimation for the value of protein expression of NFκB (p50) was 0.64 (sign. = 0.000). It shows that if the protein expression of NFκB (p50) of the respondents is incremented by 1 (one) unit, the risk of chronic periodontitis increases 1.64 times. Box plot diagram shows that the distribution of the protein expression of NFκB (p50) between patients with aggressive periodontitis and chronic periodontitis patients is significantly different. In NF-κB (p65) the Z value was -7.137 and significance of 0000, with α= 5%, showed significant differences in protein expression of NF-κB (p65) between patients with aggressive periodontitis and chronic periodontitis. OR estimates for protein expression of NFκB (p65) was 0.66 (sign. = 0.000). This indicates that if the protein expression of NFκB (p65) respondents is incremented by 1 (one) unit, the risk of chronic periodontitis increases 1.5 times. Box plot diagram shows that the distribution of the protein expression of NFκB (p65) between patients with aggressive and chronic periodontitis patients is significantly different. Conclusion: The protein expression of NF-κB (p50/p65) has more influence on the incidence of chronic periodontitis patients, so it can be used as a marker for chronic periodontitis.