Open AccessAssessment of Cytogenetic Damage to Exfoliated Gingival Cells in Patients with Chronic Generalized Periodontitis
Author(s) -
Antonija Tadin,
Lidia Gavić,
Marija Roguljić,
Mario Babić,
Ivan Galić,
Davor Želježić
Publication year - 2021
Publication title -
acta clinica croatica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.274
H-Index - 20
eISSN - 1333-9451
pISSN - 0353-9466
DOI - 10.20471/acc.2021.60.02.06
Subject(s) - micronucleus test , periodontium , chronic periodontitis , periodontitis , medicine , micronucleus , dentistry , aggressive periodontitis , pathology , toxicity
The aim of this cross-sectional study was to investigate the occurrence of chromosomal abnormalities through the frequency of micronuclei and other genomic damage markers in patients with chronic generalized periodontitis and without periodontal disease. Micronucleus assay was performed in exfoliated gingival epithelial cells of 35 patients with generalized chronic periodontitis and 30 control subjects with healthy periodontium. Full mouth clinical examination was performed to define periodontal condition. The mean number of cells with micronuclei observed in chronic periodontitis and control groups was 1.8 (±1.49) and 2.0 (±1.34), respectively. Differences between the groups were not significant (p=0.574). Compared to control subjects, patients with chronic periodontitis showed a significant increase in the number of binucleated cells (p≤0.001) and number of cells with nucleoplasmic bridges (p=0.042). Study results indicated that chronic periodontitis was not associated with higher occurrence of chromosomal damage in gingival cells compared to individuals with healthy periodontium.