Open AccessCirculating tumor cells: real-time monitoring of the course of breast cancer
Author(s) -
Alena V. Zyuzyukina,
М. О. Ватрушкина,
Р. А. Зуков
Publication year - 2021
Publication title -
sibirskoe medicinskoe obozrenie
Language(s) - English
Resource type - Journals
eISSN - 2500-0136
pISSN - 1819-9496
DOI - 10.20333/25000136-2021-2-94-96
Subject(s) - aptamer , circulating tumor cell , breast cancer , medicine , metastatic breast cancer , cancer , peripheral blood , disease monitoring , oncology , pathology , cancer cell , disease , cancer research , metastasis , biology , microbiology and biotechnology
The aim of the research. Th is study aims to determine the content of circulating tumor cells (CTCs) in the peripheral blood using the MDA 231 aptamer in the dynamics of the disease. Material and methods. Th e study included 6 patients with breast cancer, who have been under dispensary supervision since 2017. Detection of CTCs was carried out in 3.5 ml of the blood of BC patients with the help of the MDA 231 aptamer which is affi ne for breast cancer cells, labeled with the fl uorescent Cy-5 dye. Th e count of CTCs in the blood samples was performed using fl uorescent and laser scanning microscopy. Results. Using the MDA231 aptamer, the content of CTCs in the peripheral blood of patients with breast cancer in the dynamics of the disease was analyzed. CTCs were detected in 1 patient, who was subsequently diagnosed with metastatic liver damage. Conclusion. Th e study demonstrates the possibility of using aptamers to determine the risk of relapse and to evaluate the eff ectiveness of antitumor therapy in patients with breast cancer.