Open AccessStructure approaches to study of DNA aptamers in solution
Author(s) -
Roman V. Moryachkov,
P.A. Nikolaeva,
В. А. Спиридонова
Publication year - 2021
Publication title -
sibirskoe medicinskoe obozrenie
Language(s) - English
Resource type - Journals
eISSN - 2500-0136
pISSN - 1819-9496
DOI - 10.20333/25000136-2021-2-76-78
Subject(s) - aptamer , nucleic acid , biomolecule , dna , nucleic acid structure , computational biology , molecule , g quadruplex , chemistry , nanotechnology , biological system , biology , rna , materials science , genetics , biochemistry , organic chemistry , gene
The high potential of aptamers – specifi c molecular agents based on short single-stranded nucleic acids – makes high demands on the molecules under development for the effi ciency of interaction with target biomolecules. In this work, approaches are considered for studying the spatial structure of DNA aptamers in solution using various complementary methods, which make it possible to obtain a more complete picture of the formation of the structure and conformational changes, to track the interaction with the target protein, the tendency to oligomerization, and to characterize the spatial structure of both individual molecules and complexes.