Open AccessThe receptor-binding domain: aptamers (SARS-CoV-2) binding investigation by isothermal titration calorimetry
Author(s) -
Vicente Dominguez Arca
Publication year - 2021
Publication title -
sibirskoe medicinskoe obozrenie
Language(s) - English
Resource type - Journals
eISSN - 2500-0136
pISSN - 1819-9496
DOI - 10.20333/25000136-2021-2-74-75
Subject(s) - isothermal titration calorimetry , aptamer , calorimetry , covid-19 , chemistry , binding domain , biophysics , computational biology , materials science , binding site , biology , biochemistry , microbiology and biotechnology , thermodynamics , physics , medicine , disease , pathology , infectious disease (medical specialty)
The aim of the research. To optimize the calorimetry method, which allows to study the binding of the receptor-binding domain (RBD) protein of the coronavirus COVID-19 to aptamer 27 obtained using a computational method. Material and methods. Computational modeling and calorimetry methods were used to optimize the method. Results. Th e results showed that the calorimetry method makes it possible to assess the binding of the RBD protein to the aptamer. Conclusion. Th ese experiments will be run as soon as will be possible to determine the optimal conditions to functionalize gold substrates with the previously thiolated aptamers.