Open AccessCharacterization Physiology and Molecular Bacteria Symbiont- Entomopathogenic Nematodes based of Gene Sequences Encoding the 16S rRNA District of Bromo Probolinggo
Author(s) -
Bagus Setiawan,
Didik Sulistyanto,
Kartika Senjarini
Publication year - 2017
Publication title -
jurnal ilmu dasar
Language(s) - English
Resource type - Journals
ISSN - 2442-5613
DOI - 10.19184/jid.v18i1.2723
Subject(s) - biology , 16s ribosomal rna , bacteria , microbiology and biotechnology , bacillus cereus , gene , sequence analysis , genomic dna , strain (injury) , cereus , ribosomal rna , genetics , anatomy
This study aims to identify entomopathogenic nematodes symbiotic bacteria phenotypically and based on the gene encoding 16S rRNA sequences. Bacterial symbionts of entomopathogenic nematodes, obtained from isolates from the area Wonokerto (WN01) and isolates Sukapura (SP01), Bromo, Probolinggo, two symbiont bacteria was found in entomopathogenic nematodes Steinernema sp. The method used in this study are: the isolation of entomopathogenic nematodes Steinernema sp. and bacterial symbionts conventionally for the identification of phenotypically, after the characterization of bacterial isolates, the isolation of genomic DNA, 16S rRNA PCR, DNA purification and DNA sequence analysis. The results based on phenotypic characterization showed that isolates WN01 and SP01, yellowish white, gram positive, negative bioluminenscene, catalase positive, can not hydrolyze urea, and also can not produce H2S. The results of the gene encoding 16S rRNA sequence can be deduced WN01 isolates have in common with the bacteria Bacillus strain toyonensis BCT 7112, while the SP01 isolates have in common with the bacteria Bacillus strain cereus ATCC 14 579.