Open AccessPurification of Xylooligosaccharide From Casavva Pulp by Ultrafiltration Method
Author(s) -
Anak Agung Istri Ratnadewi,
Kamelia Rizqi Fauziyah,
Dwi Indarti
Publication year - 2021
Publication title -
berkala sainstek
Language(s) - English
Resource type - Journals
ISSN - 2339-0069
DOI - 10.19184/bst.v9i2.23388
Subject(s) - pulp (tooth) , chemistry , chromatography , xylan , ultrafiltration (renal) , cellulose , xylanase , hemicellulose , densitometry , enzymatic hydrolysis , hydrolysis , gel permeation chromatography , xylose , thin layer chromatography , size exclusion chromatography , food science , biochemistry , enzyme , fermentation , polymer , organic chemistry , medicine , physics , pathology , quantum mechanics
Xylan is the main component of hemicellulose. Xylan can be extracted from agricultural waste, such as cassava pulp. Xylan is used as an endo-β-1,4-D-xylanase substrate to produce impure xylooligosaccharides (XOS)This study aims to purify XOS from cassava pulp using the ultrafiltration method. The components of XOS obtained from the enzymatic hydrolysis were analyzed using thin layer chromatography (TLC) and densitometry methods. In addition, the XOS was purified by the ultrafiltration method using a cellulose membrane with a Molecular Weight Cut Off (MWCO) of 12 kDa. The permeate obtained from the purification results was also analyzed using TLC and densitometry. The results of this study indicated that the components in XOS cassava pulp before and after purification by the TLC method were X5 and X6, while the XOS components before and after purification by the densitometric method were X3, X4 and X5.