Open AccessPuces à ADN comme outil d'identification moléculaire pour <em>Culicoides</em>
Author(s) -
J. C. De Witte,
Isra Deblauwe,
G. De Deken,
R. De Deken,
Maxime Madder,
R. Meiswinkel
Publication year - 2009
Publication title -
revue d'élevage et de médecine vétérinaire des pays tropicaux/revue d'élevage et de médecine vétérinaire des pays tropicaux
Language(s) - English
Resource type - Journals
eISSN - 1951-6711
pISSN - 0035-1865
DOI - 10.19182/remvt.10054
Subject(s) - culicoides , microarray , biology , polymerase chain reaction , genotype , microbiology and biotechnology , virology , genetics , gene , gene expression , zoology
A DNA microarray test based on internal transcribed spacer 1 (ITS1) genotype expression was developed to identify Culicoides species of Northwestern Europe belonging to the Culicoides Obsoletus complex. The assay was designed so as to allow interpretation by the naked eye. False positive and false negative results were eliminated. The need for expensive laboratory equipment and reagents was kept as low as possible, making the technique affordable and feasible for any diagnostic laboratory with polymerase chain reaction (PCR) facilities. The microarray test could be validated through the three ringtests organised by Medreonet. Use of this microarray can improve monitoring adult and immature Culicoides species.