Open AccessTaux d'infection de Culicoides imicola par différents sérotypes du virus de la fièvre catarrhale ovine après contamination par voie orale
Author(s) -
R. Del Río,
Gert J. Venter,
Miguel Ángel Miranda,
Claudia Paredes-Esquivel,
Javier Lucientes,
C. Calvete,
Rolando Estrada
Publication year - 2009
Publication title -
revue d'élevage et de médecine vétérinaire des pays tropicaux/revue d'élevage et de médecine vétérinaire des pays tropicaux
Language(s) - English
Resource type - Journals
eISSN - 1951-6711
pISSN - 0035-1865
DOI - 10.19182/remvt.10044
Subject(s) - biology , serotype , virology , virus , baby hamster kidney cell , vaccination , veterinary medicine , medicine
Bluetongue (BT) is an infectious disease that is spreading northwards in Europe. Knowing the infection rates of the different virus serotypes present in a region and of those that have the potential to enter that region is critical to respond adequately to the disease and set up preventive measures such as vaccination. The present study shows that wild-caught South African Culicoides imicola Kieffer (Diptera: Ceratopogonidae) can become infected with and permit the replication of the different strains of BT virus serotypes detected and isolated in Spain (BTV-1, 2, 4 and 8). Virus replication was measured over time by assaying individual midges on baby hamster kidney (BHK)-21 cells using a microtitration procedure. The mean prevalence of BTV infection after 10 days’ extrinsic incubation (DEI) at 23.5ºC was lower than 1% in all four serotypes. The virus concentration of individual C. imicola infected with BTV ranged from 1.4 to 3.9 TCID50. BTV titres higher than 2.5 log10 TCID50 found in individual C. imicola suggest that this species may be able to transmit that viral strain to susceptible hosts.