Open AccessEvaluation in vitro of antimicrobial activity of basil extract (Ocimum basilicum L.) on an acrylic surface of removable orthodontic appliances
Author(s) -
Jéssica Fernanda Sedenho,
Vanessa Ferreira Moraes,
Adilson César de Abreu Bernardi,
Nádia Lunardi,
Eloísa Marcantônio Boeck
Publication year - 2014
Publication title -
journal of research in dentistry
Language(s) - English
Resource type - Journals
ISSN - 2317-5907
DOI - 10.19177/jrd.v2e32014227-234
Subject(s) - ocimum , basilicum , serial dilution , antimicrobial , antibacterial activity , dilution , sonication , chlorhexidine , biofilm , chemistry , microbiology and biotechnology , materials science , food science , traditional medicine , chromatography , dentistry , biology , medicine , bacteria , alternative medicine , genetics , thermodynamics , physics , pathology
AIM: The aim of this study was to evaluate in vitro the antimicrobial activity of Ocimum basilicum L. (basil) extract in S. Muttans biofilm colonized in specimens confectioned in the same acrylic used for removable orthodontics appliances. MATERIAL AND METHODS: To perform this work, 42 specimens were confectioned (sterile spherical acrylic disks) and immersed in pure extract and in serial dilution of extract (1:2 until 1:10) during 24, 48 and 72 hours. For each time of exposition, a disintegration of bacterial film by sonication in saline and posterior seed in agar, for colony count, were carried out. ATCC strains of S. muttans were selected, and 2% chlorhexidine solution was used as inhibition control. RESULTS: The results showed, by quantitative analysis, that basil extract has antibacterial activity in S. muttans biofilm, when used in pure state or in dilution until 1:4. CONCLUSION: Besides, it was possible observe the more increase the incubation time, independent the dilution, the higher degradation of extract.