Open AccessDETERMINATION OF FECES IN TRACES ON MATERIAL EVIDENCE BY THE METHOD OF ASCENDANT THIN LAYER CHROMATOGRAPHY
Author(s) -
A. P. Chetvertnova,
Andrej Fedorovtsev,
Н. С. Эделев
Publication year - 2018
Publication title -
sudebnaâ medicina
Language(s) - English
Resource type - Journals
eISSN - 2411-8729
pISSN - 2409-4161
DOI - 10.19048/2411-8729-2018-4-4-30-32
Subject(s) - feces , thin layer chromatography , chromatography , distilled water , chemistry , reagent , urine , biology , microbiology and biotechnology , biochemistry
The article presents the results of a study of the pigment composition of feces in traces on material evidence by the method of thin-layer chromatography. The aim of the study was to develop a method for determining the presence of feces based on the detection of stercobilin. The objects of the study were samples of feces of adults without visible pathological changes in the liver, gallbladder, intestines in a sectional study, with a fence age of 3 days to 1 year. When using chromatography of fecal samples in the solvent system butanol-distilled water-glacial acetic acid (4:1:2) and subsequent processing with Ehrlich reagent positive results were obtained – the appearance of red stripes on chromatograms at Rf = 0,55–0,6, which indicates the presence of stercobilin. When studying the samples of meconium, blood, salvia, sweat, vaginal contents, and urine by this way, the reaction to the presence of stercobilin was negative. Thus, this method can be used in practice to determine the presence of feces in the traces on physical evidence.