Open AccessMise Au Point D’un Protocole De Sterilisation D’explants Nodaux D’alchornea Cordifolia Avec De L’acide Triclororoisocyanurique
Author(s) -
Aurélien Mokea-Niaty,
Samson Daudet Medza Mve,
Alexis Nicaise Lepengué,
Antoine Mitte Mbeang Beyeme,
Christian Moupela,
Maurice Ognalaga,
Darlène Badjina Eko,
N. S. Mustafa,
Bertrand M’Batchi
Publication year - 2017
Publication title -
european scientific journal
Language(s) - English
Resource type - Journals
eISSN - 1857-7881
pISSN - 1857-7431
DOI - 10.19044/esj.2017.v13n15p274
Subject(s) - explant culture , trichloroisocyanuric acid , calcium hypochlorite , carbendazim , disinfectant , sterilization (economics) , chemistry , fungicide , horticulture , sodium hypochlorite , chlorothalonil , chlorine , traditional medicine , botany , biology , in vitro , medicine , biochemistry , organic chemistry , economics , monetary economics , foreign exchange market , foreign exchange
Trichloroisocyanuric acid is a swimming pool disinfectant and is readily accessible. As a result, there is the need to use it as a substitute for conventional disinfectants in in vitro culture. Nodal explants of Alchornea cordifolia, harvested in a natural environment, have been rinsed abundantly with Dettol under running water. Then it was soaked in Talo Plus (550 g/l carbendazim and 100 g/l Chlorothalonil) at 5 ml/liter, which is a broad spectrum fungicide. After then, it was immersed in 70% alcohol for 10 minutes before being soaked in different solutions of trichloroisocyanuric acid to: 6, 4, 3, 2, 1, 0.3, 0.1, and 0.08%. The explants were disinfected completely of all contaminating bacterial and fungal exogenous. This was after a treatment in solutions of acidic trichloroisocyanurique of 6 to 0.08%. The results showed that the losses of active chlorine remained low during storage at temperatures of 4 to 18 ± 2°C. They reach only 5.29% after 72 hours. At room temperature of 27 ± 2 ° C, these losses are more than 30% after three days. Concentrations of 0.1 to 0.3% are effective for the disinfection of explants. This protocol of explants disinfection in vitro culture could therefore be advantageously substituted using the hypochlorite of calcium or the chloride of mercury.