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Obesity and periodontitis: An experimental study to evaluate periodontal and systemic effects of comorbidity
Author(s) -
Virto Leire,
Cano Pilar,
JiménezOrtega Vanesa,
FernándezMateos Pilar,
González Jerián,
Esquifino Ana Isabel,
Sanz Mariano
Publication year - 2018
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2017.170355
Subject(s) - periodontitis , medicine , porphyromonas gingivalis , resistin , adiponectin , endocrinology , adipokine , obesity , leptin , gastroenterology , insulin resistance
Background Obesity and overweight have been associated with periodontitis. This study aims to evaluate periodontal and systemic effects of this association in a validated experimental model. Methods Twenty‐eight male Wistar rats were randomly divided into four groups: 1) control group (Con) (fed with standard diet); 2) high‐fat diet group (HFD) (fed with a diet containing 35.2% fat); 3) control group with induced periodontitis (Con‐Perio); and 4) HFD group with induced periodontitis (HFD‐Perio). To induce periodontitis, oral gavages with Porphyromonas gingivalis ATCC W83K1 and Fusobacterium nucleatum DMSZ 20482 were used. Periodontal outcomes were evaluated by inflammatory parameters, periodontal probing depth (PD), and modified gingival index (MGI). Systemic effects were evaluated by measuring levels of inflammatory cytokines, insulin, adiponectin, and leptin using multiplex immunoassays and levels of visfatin, resistin, lipid profiles, transaminases, and plasma endotoxin using colorimetric tests and the glucose tolerance test. Results Clinical parameters (PD and MGI) were significantly increased ( P < 0.05) in the induced periodontitis groups compared with controls. The HFD‐Perio group demonstrated significantly higher PD compared with Con‐Perio group. Lipid profiles, cytokines, and adipocytokines showed significantly elevated levels in the HFD‐Perio group compared with the other groups. Similarly, glucose levels in the HFD‐Perio group were significantly higher ( P < 0.05) than in the HFD group, and hepatic damage parameters demonstrated a tendency toward higher levels in the HFD‐Perio group. Conclusion Obesity and periodontitis demonstrated a comorbidity effect on both systemic inflammatory and metabolic dysregulation biomarkers, with increased glucose, dyslipidemia and hepatic damage.