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Postoperative Weight Loss Masks Metabolic Impacts of Periodontitis in Obese Rodents
Author(s) -
Yu Ting,
Zhao Li,
Huang Xin,
Xie Meilian,
Wang Xiangli,
Ma Chanjuan,
Xu Yixin,
Wang Yixiong,
Xie Baoyi,
Luo Gang,
Zhang Jincai,
Xuan Dongying
Publication year - 2017
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2017.160655
Subject(s) - periodontitis , confounding , medicine , triglyceride , obesity , insulin resistance , endocrinology , homeostatic model assessment , cholesterol , high density lipoprotein , ligation , diabetes mellitus , glucose homeostasis
Background: Postoperative weight loss (POWL) is expected to occur in combined models of obesity and periodontitis. This study explores the confounding effects of POWL on the impact of ligation‐induced periodontitis on glucose and lipid metabolism in obese animals. Methods: Combined mouse models of diet‐induced obesity (DIO) and ligation‐induced periodontitis (5‐ or 10‐day ligation) were studied. Fasting serum glucose (FSG), fasting insulin (Fins), and lipids including triglyceride (TG), total cholesterol (TC), and low‐ and high‐density lipoprotein cholesterol (HDLC), were detected via biochemistry and enzyme‐linked immunosorbent assay. POWL and homeostasis model assessment of insulin resistance (HOMA‐IR) were calculated. Analysis of covariance was performed to identify confounding effects of POWL. Results: The obesity, periodontitis, and 10‐day groups exhibited greater POWL than corresponding controls ( P <0.01). Without considering POWL, conflicting results were found, including: 1) contradictory changes in HDLC caused by obesity or periodontitis; and 2) unequal levels of FSG, TC, and HDLC between days 5 and 10 in the sham‐ligation controls. Moreover, upregulating effects of periodontitis were found only on TG in the DIO mice, whereas those on Fins, HOMA‐IR, and HDLC were statistically veiled. After the confounding effects of POWL were filtered, periodontitis promoted increased levels of not only TG but also Fins, HOMA‐IR, and HDLC in the DIO mice ( P <0.05). Conclusions: When analyzing the interrelationship between obesity and periodontitis, the confounding effects of an imbalanced POWL should be considered. Otherwise, impact of periodontitis on metabolic dysregulation in obese animals may be underestimated.