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Angiotensin II Type 1 Receptor Knockdown Impairs Interleukin‐1β‐Induced Cytokines in Human Periodontal Fibroblasts
Author(s) -
Gabriele Lilian Gobbo,
Morandini Ana Carolina,
Dionísio Thiago José,
Santos Carlos Ferreira
Publication year - 2017
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2016.160354
Subject(s) - periodontal fiber , angiotensin ii , gene knockdown , receptor , interleukin , chemistry , immunology , medicine , cytokine , dentistry , biochemistry , apoptosis
Background: The renin‐angiotensin (Ang) system (RAS) has been reported as an important modulator of inflammatory and immune responses. Evidence suggests an alternative Ang 1‐7/Mas receptor axis as counter‐regulatory to the classic RAS Ang II/Ang II Type 1 (AT1) receptor axis. It is known that periodontal pathogens elicit host‐derived immune response due to release of cytokines such as interleukin (IL)‐1β, and fibroblasts are among the most numerous sentinel cells that contribute to this production. The aim of this study is to determine whether AT1 receptor (AT1R) contributes to production of inflammatory cytokines that are important for periodontal pathogenesis using primary human gingival fibroblasts (HGFs) and human periodontal ligament fibroblasts (HPLFs) stimulated with IL‐1β. Methods: Through RNA interference or pharmacologic inhibition using AT1R antagonist losartan, HGF and HPLF were stimulated by IL‐1β for 3 (messenger RNA [mRNA]) or 24 (protein) hours. Results: IL‐1β upregulated mRNA expression of AT1R, IL‐1β, IL‐6, IL‐8, tumor necrosis factor‐alpha, and osteoprotegerin (OPG) in HGF and HPLF. AT1R knockdown impaired IL‐1β‐induced IL‐6 and IL‐8 secretion in cultured HGF and HPLF. AT1R silencing also increased OPG gene expression in HGF only. Pharmacologic inhibition of AT1R through losartan modulated mRNA transcription of IL‐6 and IL‐8 in HPLF but not in HGF. In contrast, IL‐1β‐induced secretion of IL‐6 and IL‐8 was not influenced by losartan in HGF or HPLF. Conclusion: These results suggest that AT1R knockdown and AT1R pharmacologic blockade by losartan may differently control balance of inflammatory cytokines, such as IL‐6 and IL‐8, in primary human periodontal fibroblasts.