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Dry Extract of Matricaria recutita L. (Chamomile) Prevents Ligature‐Induced Alveolar Bone Resorption in Rats via Inhibition of Tumor Necrosis Factor‐α and Interleukin‐1β
Author(s) -
Guimarães Mariana Vasconcelos,
Melo Iracema Matos,
Adriano Araújo Vilana Maria,
Tenazoa Wong Deizy Viviana,
Roriz Fonteles Cristiane Sá,
Moreira Leal Luzia Kalyne Almeida,
Ribeiro Ronaldo Albuquerque,
Lima Vilma
Publication year - 2016
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2016.150411
Subject(s) - osteoprotegerin , medicine , endocrinology , bone resorption , myeloperoxidase , rankl , osteoclast , dental alveolus , acid phosphatase , alkaline phosphatase , chemistry , inflammation , activator (genetics) , biochemistry , receptor , dentistry , enzyme
Background: Matricaria recutita L. (chamomile) has demonstrated anti‐inflammatory activity. Accordingly, the ability of the Matricaria recutita extract (MRE) to inhibit proinflammatory cytokines and its influence on alveolar bone resorption (ABR) in rats. Methods: Wistar rats were subjected to ABR by ligature with nylon thread in the second upper‐left molar, with contralateral hemiarcade as control. Rats received polysorbate TW 80 (vehicle) or MRE (10, 30, and 90 mg/kg) 1 hour before ligature and daily until day 11. The periodontium was analyzed by macroscopy, histometry, histopathology, and immunohistochemistry for the receptor activator of nuclear factor‐kappa B ligand (RANKL), osteoprotegerin (OPG), and tartrate‐resistant acid phosphatase (TRAP). The gingival tissue was used to quantify the myeloperoxidase (MPO) activity and tumor necrosis factor (TNF)‐α and interleukin (IL)‐1β levels by enzyme‐linked immunosorbent assay. Blood samples were collected to evaluate bone‐specific alkaline phosphatase (BALP), leukogram, and dosages of aspartate and alanine transaminases, urea, and creatinine. Aspects of liver, kidneys, spleen, and body mass variations were also evaluated. Results: The 11 days of ligature induced bone resorption, low levels of BALP, leukocyte infiltration; increase of MPO, TNF‐α, and IL‐1β; immunostaining increase for RANKL and TRAP; reduction of OPG and leukocytosis, which were significantly prevented by MRE, except for the low levels of BALP and the leukocytosis. Additionally, MRE did not alter organs or body weights of rats. Conclusion: MRE prevented the inflammation and ABR by reducing TNF‐α and IL‐1β, preventing the osteoclast activation via the RANKL–OPG axis, without interfering with bone anabolism.