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Porphyromonas gingivalis and Epstein‐Barr Virus Are Associated With Increased Levels of Visfatin in Gingival Crevicular Fluid
Author(s) -
Özcan Erkan,
Saygun N. Işıl,
Serdar Muhittin A.,
Kubar Ayhan,
Bengi V. Umut
Publication year - 2016
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2015.150424
Subject(s) - porphyromonas gingivalis , prevotella intermedia , periodontitis , medicine , chronic periodontitis
Background: There is little clinical information on the relationship between periodontopathogens and visfatin. The purpose of this study is to determine visfatin levels in the gingival crevicular fluid (GCF) of healthy individuals and patients with periodontitis and to investigate the possible relationship between this adipokine and the presence and levels of Porphyromonas gingivalis , Prevotella intermedia , Prevotella nigrescense , and Epstein‐Barr virus (EBV). Methods: Eighteen healthy individuals and 27 patients with periodontitis were included in this study. GCF and plaque samples were obtained from all individuals. Visfatin levels were analyzed by enzyme‐linked immunosorbent assay, and the bacterial numbers were evaluated by the reverse transcription‐polymerase chain reaction method. Results: In patients with periodontitis, the visfatin levels in the GCF (mean: 84.29 ng/mL; range: 63.8 to 108.9 ng/mL) were significantly higher compared with those of the healthy individuals (mean: 38.06 ng/mL; range: 13.8 to 89.02 ng/mL) ( P < 0.01). There was a positive correlation between the visfatin levels and P. gingivalis ( r = 0.266, P < 0.05), whereas no correlation was found between visfatin levels and other microorganisms. In addition, the visfatin levels were found to be higher in individuals in whom P. gingivalis was detected than for those without P. gingivalis ( P < 0.01). The visfatin levels were also found to be higher in individuals in whom EBV was detected ( P < 0.05). Conclusions: To the best of the authors’ knowledge, the present study is the first one to show the correlation of periodontopathogens and GCF visfatin levels. P. gingivalis colonization of the periodontal pockets may increase visfatin secretion. Furthermore, the presence of EBV in the plaque may be another factor that causes an increase in visfatin levels.