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Pilot Study on Oral Health Status as Assessed by an Active Matrix Metalloproteinase‐8 Chairside Mouthrinse Test in Adolescents
Author(s) -
Heikkinen Anna Maria,
Nwhator Solomon O.,
Rathnayake Nilminie,
Mäntylä Päivi,
Vatanen Päivi,
Sorsa Timo
Publication year - 2016
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2015.150377
Subject(s) - medicine , periodontitis , dentistry , immunoassay , point of care testing , biomarker , collagenase , matrix metalloproteinase , chronic periodontitis , pathology , immunology , antibody , chemistry , enzyme , biochemistry
Background: Matrix metalloproteinase (MMP)‐8 is a major destructive collagenase involved in periodontitis and can be regarded as a periodontitis biomarker. A neutrophil collagenase 2 (active MMP‐8 [aMMP‐8]) oral fluid immunoassay has recently been demonstrated to be a periodontitis risk indicator among adults. The aim of this study is to investigate whether a point‐of‐care mouthrinse test based on an aMMP‐8 immunoassay could identify patients with oral inflammatory burden (periodontitis and caries) among adolescents with early pathologic findings. Methods: This cross‐sectional study was carried out at the Kotka Health Center, Finland. First, the aMMP‐8 chairside mouthrinse test was performed on enrolled individuals (adolescents aged 15 to 17 years, n = 47), and the results were read based on a color change within 5 minutes. Then, full‐mouth clinical parameters of oral health were assessed, including periodontal, oral mucosal, and caries status. Results: The sensitivity and specificity of the test for bleeding on probing were 71.8% and 77.5%, respectively ( P = 0.05); for ≥1 site with probing depth (PD) ≥4 mm, 48.3% and 100% ( P <0.001); for ≥2 sites with PD ≥4 mm, 63.6% and 100% ( P <0.001); and for >2 sites with PD ≥4 mm, 76.5% and 96.7% ( P <0.01). Regarding periodontitis (≥1 site with PD ≥4 mm), hardly any false‐positive results were identified. The sensitivity of the immunoassay for ≥1 caries lesions was 76.5%, and the specificity was 96.7% ( P <0.01). Conclusions: In 5 minutes, the aMMP‐8 chairside test showed promising results, recognizing oral inflammatory burden in adolescents with early initial signs of periodontitis. Caries lesions could also be detected, but less efficiently.