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Effect of Doxycycline in Gel Form on Bone Regeneration: Histomorphometric and Tomographic Study in Rat Calvaria
Author(s) -
Silva Amanda C.,
Oliveira Marina R.,
Amaral Lysandro F. A.,
Ferreira Sabrina,
Garcia Idelmo R.,
Mariano Ronaldo C.
Publication year - 2016
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2015.150343
Subject(s) - calvaria , doxycycline , analysis of variance , bone density , regeneration (biology) , chemistry , medicine , biology , antibiotics , osteoporosis , biochemistry , in vitro , microbiology and biotechnology
Background: This study aims to investigate the effects of the local application of doxycycline in the form of natrosol‐based gel on bone regeneration by examining critical defects in rat calvaria. Methods: Twenty‐four rats were randomly divided into three groups with eight animals each, according to the treatment received: BC (untreated, filled with blood clot), NAT (natrosol gel alone), and DOX (10% doxycycline gel). Four animals from each group were euthanized at 4 and 8 weeks postoperatively. In tomographic analysis, mean density in the region of the defect was calculated as a percentage relative to the native bone density. In histomorphometric analysis, the newly formed bone area was calculated as a percentage of the total area. The values obtained underwent analysis of variance and Tukey testing ( P <0.05). Results: The mean percentage of tomographic density in the region of the defect at the end of 8 weeks was higher for DOX (44.37%), and statistical differences in this period were observed between DOX and NAT (33.10%) and DOX and BC (32.43%). Regarding new bone formation, at the end of 8 weeks, DOX (61.11%) also had the highest mean bone formation, and statistical differences were observed between DOX and NAT (34.61%) and DOX and BC (23.11%). Conclusion: It was observed that 10% doxycycline gel had a good effect on bone regeneration regarding the filling of critical defects in rat calvaria.