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Alveolar Bone Resorption Induced by CD4 + CD45RB High‐Density T‐Cell Transfer in Immunocompromised Mice
Author(s) -
Park JungChul,
Kim BoKyoung,
Jung ImHee,
Choi Eunyoung,
Kim ChangSung
Publication year - 2014
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2014.140077
Subject(s) - chemistry , adoptive cell transfer , resorption , t cell , pathology , bone resorption , stromal cell , transplantation , dental alveolus , bone marrow , medicine , immunology , dentistry , immune system
Background: The aims of this study are to determine whether the antigen‐inexperienced (naive, CD45RB high‐density) T‐cell (CD4 + CD45RB High T‐cell) transfer model is associated with alveolar bone resorption, to elucidate the local osteogenic/adipogenic potential of alveolar bone marrow stromal cells (ABCs) from T‐cell–transferred animals, and to investigate the systemic osteogenic potential by transplanting human periodontal ligament stem cells (hPDLSCs) into these animals. Methods: CD4 + CD45RB High and CD4 + CD45RB Low (antigen‐experienced [memory, CD45RB low‐density]) T cells were sorted and transferred into severe combined immunodeficiency (SCID) mice to induce inflammatory bowel disease–like syndrome (n = 8). hPDLSCs were transplanted into T‐cell–transferred SCID mice to examine ectopic cementum formation 8 weeks after T‐cell transfer. The mandibles and tibias of these mice were retrieved for microcomputed tomography (micro‐CT), histomorphometric analysis, and isolation of ABCs 16 weeks after T‐cell transfer. The in vitro osteogenic and adipogenic potentials of the ABCs were evaluated. Results: Histologic and micro‐CT analysis revealed that the transfer of CD4 + CD45RB High T‐cell subset was sufficient for alveolar bone resorption and affected the osteogenic/adipogenic potential of ABCs. Furthermore, it was found that CD4 + CD45RB High T‐cell–transferred animals have decreased systemic osteogenic potential, as evidenced using the in vivo ectopic hPDLSC transplantation model. Conclusion: CD4 + CD45RB High T‐cell transfer induced both alveolar bone resorption and reduced systemic osteogenic potential, with a concomitant downregulation of the osteogenic potential of ABCs.