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Characterization of Highly Osteoblast/Cementoblast Cell Clones From a CD105‐Enriched Periodontal Ligament Progenitor Cell Population
Author(s) -
Saito Miki T.,
Salmon Cristiane R.,
Amorim Bruna R.,
Ambrosano Gláucia M.B.,
Casati Márcio Z.,
Sallum Enilson A.,
Nociti Francisco H.,
Silvério Karina G.
Publication year - 2014
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2014.130461
Subject(s) - progenitor cell , population , runx2 , cell , alkaline phosphatase , biology , cementoblast , microbiology and biotechnology , cellular differentiation , periodontal fiber , osteoblast , cell culture , stem cell , chemistry , in vitro , genetics , biochemistry , pathology , medicine , gene , enzyme , cementum , environmental health , dentistry , dentin
Background: It is known that periodontal ligament (PDL) harbors a heterogeneous progenitor cell population at different stages of lineage commitment. However, characterization of PDL stem cells committed to osteoblast/cementoblast (O/C) differentiation remains to be elucidated. The present study is carried out to isolate single cell–derived, cluster of differentiation (CD)105–positive PDL clones and to characterize the clones that present high potential to differentiate toward O/C phenotype in vitro. Methods: Isolation of single cell–derived colonies (clones) from a CD105‐enriched PDL progenitor cell population was performed by the ring‐cloning technique. Cell clones were evaluated for their O/C differentiation potential, metabolic activity, and expression of STRO‐1 protein. Additionally, the clones that showed potential to O/C differentiation were characterized by quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR) for expression of runt‐related transcriptor factor 2 ( RUNX2 ), alkaline phosphatase, CD105 , and CD166 during osteogenic induction. Results: Six PDL‐CD105 + clones were obtained, three being highly O/C clones (C‐O) and three others that did not have the ability to produce mineralized matrix in vitro (C‐F). The C‐O group showed lower metabolic activity compared with the C‐F group, and both cell groups were positively immunostained for STRO‐1. qRT‐PCR analysis demonstrated an increased expression of transcripts for RUNX2 and CD166 during the maturation of C‐O cells toward O/C phenotype. Conclusions: These results provide evidence that PDL‐CD105 + purified progenitor cells comprise a heterogeneous cell population that presents a cell subset with high O/C potential and, further, that surface antigen CD166 is modulated during the O/C maturation of this cell subset.