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Impact of Periodontal Therapy on the Subgingival Microbiota of Severe Periodontitis: Comparison Between Good Responders and Individuals With Refractory Periodontitis Using the Human Oral Microbe Identification Microarray
Author(s) -
Colombo Ana Paula V.,
Bennet Susan,
Cotton Sean L.,
Goodson J. Max,
Kent Ralph,
Haffajee Anne D.,
Socransky Sigmund S.,
Hasturk Hatice,
Van Dyke Thomas E.,
Dewhirst Floyd E.,
Paster Bruce J.
Publication year - 2012
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2012.110566
Subject(s) - tannerella forsythia , fusobacterium nucleatum , prevotella intermedia , microbiology and biotechnology , periodontitis , porphyromonas gingivalis , veillonella , chronic periodontitis , capnocytophaga , treponema denticola , medicine , prevotella , fusobacterium , biology , dentistry , streptococcus , bacteroides , pathology , bacteria , honeysuckle , genetics , alternative medicine , traditional chinese medicine
Background: This study compares the changes to the subgingival microbiota of individuals with “refractory” periodontitis (RP) or treatable periodontitis (good responders [GR]) before and after periodontal therapy by using the Human Oral Microbe Identification Microarray (HOMIM) analysis. Methods: Individuals with chronic periodontitis were classified as RP (n = 17) based on mean attachment loss (AL) and/or >3 sites with AL ≥2.5 mm after scaling and root planing, surgery, and systemically administered amoxicillin and metronidazole or as GR (n = 30) based on mean attachment gain and no sites with AL ≥2.5 mm after treatment. Subgingival plaque samples were taken at baseline and 15 months after treatment and analyzed for the presence of 300 species by HOMIM analysis. Significant differences in taxa before and post‐therapy were sought using the Wilcoxon test. Results: The majority of species evaluated decreased in prevalence in both groups after treatment; however, only a small subset of organisms was significantly affected. Species that increased or persisted in high frequency in RP but were significantly reduced in GR included Bacteroidetes sp., Porphyromonas endodontalis , Porphyromonas gingivalis , Prevotella spp., Tannerella forsythia , Dialister spp., Selenomonas spp., Catonella morbi , Eubacterium spp., Filifactor alocis , Parvimonas micra , Peptostreptococcus sp. OT113, Fusobacterium sp. OT203, Pseudoramibacter alactolyticus , Streptococcus intermedius or Streptococcus constellatus , and Shuttlesworthia satelles. In contrast, Capnocytophaga sputigena , Cardiobacterium hominis, Gemella haemolysans , Haemophilus parainfluenzae , Kingella oralis , Lautropia mirabilis , Neisseria elongata , Rothia dentocariosa , Streptococcus australis , and Veillonella spp. were more associated with therapeutic success. Conclusion: Persistence of putative and novel periodontal pathogens, as well as low prevalence of beneficial species was associated with chronic refractory periodontitis.