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A Novel Approach to the Use of Doxycycline‐Loaded Biodegradable Membrane and EDTA Root Surface Etching in Chronic Periodontitis: A Randomized Clinical Trial
Author(s) -
Gamal Ahmed Y.,
Kumper Radi M.
Publication year - 2012
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2011.110476
Subject(s) - doxycycline , debridement (dental) , dentistry , membrane , chemistry , periodontitis , chronic periodontitis , medicine , antibiotics , biochemistry
Background: The release profile of 25% doxycycline (DOX) gel loaded on a biodegradable collagen membrane (COL) after 24% EDTA root surface etching was evaluated. Methods: Thirty systemically healthy patients, each with at least one pair of contralateral interproximal intrabony defects ≥4 mm deep, along with an interproximal probing depth ≥6 mm and clinical attachment loss ≥4 mm, were randomized into two groups. Group 1 consisted of sites treated with open‐flap debridement followed by placement of DOX gel‐loaded COL (DOX–COL), whereas group 2 sites were treated with flap surgery followed by the placement of DOX–COL after EDTA etching of the exposed root surfaces (DOX–COL + EDTA). Samples of gingival crevicular fluid were obtained 1, 3, 7, 14, and 21 days after surgery. Separation was performed, and quantitative measurements of DOX were taken with a high‐performance liquid chromatography. Clinical evaluation and follow‐up for 6 months were performed. Results: At 21 days, DOX–COL + EDTA group showed 5.3 μg/mL value. However, no DOX was detected in samples of the DOX–COL group. DOX–COL + EDTA‐treated group retained more DOX during the periods of 3, 7, 10, and 14 days than did the DOX–COL group. Conclusion: EDTA root surface etching could enhance DOX availability in the gingival crevicular fluid after its release from the collagen membrane.