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Interleukin‐8 Gene Promoter Polymorphism (rs4073) May Contribute to Chronic Periodontitis
Author(s) -
Andia Denise C.,
de Oliveira Naila F. P.,
Letra Ariadne M.,
Nociti Francisco H.,
Line Sergio R. P.,
de Souza Ana P.
Publication year - 2011
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2010.100513
Subject(s) - chronic periodontitis , periodontitis , gene , medicine , genetics , promoter , biology , dentistry , gene expression
Background: The proinflammatory chemokine interleukin (IL)‐8 is important in the regulation of the inflammatory response. Analyses of the single nucleotide polymorphism (SNP) reference sequence (rs) 4073 showed that the A allele upregulated IL‐8 levels after stimulation with lipopolysaccharides. We investigated the association of the SNP rs4073 with chronic periodontitis. Methods: Genotyping was performed by a standard polymerase chain reaction–restriction fragment length polymorphism assay in 289 genomic DNA samples of healthy control subjects and patients with chronic periodontitis; analyses were adjusted by multivariate logistic regression modeling. A real‐time polymerase chain reaction performance was used to detect levels of the IL‐8 mRNA. Results: The analysis pointed to a statistically significant association of chronic periodontitis with the heterozygous TA genotype ( P = 0.001); the results showed an increase in the frequency of the A allele in the diseased group (36% in the control group versus 48% in the periodontitis group). The higher levels of the IL‐8 mRNA were found in the periodontitis group, mainly in individuals who presented the TA genotype ( P = 0.03). Conclusion: The SNP rs4073 was associated with chronic periodontitis in non‐smoker Brazilian subjects because the frequency of the A allele was higher in the disease group than in the control group, and the TA genotype was associated with increased levels of IL‐8 mRNA transcripts.