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Phenotypic Study of Human Gingival Fibroblasts in a Medium Enriched With Platelet Lysate
Author(s) -
Naveau Adrien,
Lataillade JeanJacques,
Fournier Benjamin Philippe,
Couty Ludovic,
Prat Marie,
Ferre François Côme,
Gourven Muriel,
Durand Eric,
Coulomb Bernard,
Lafont Antoine,
Gogly Bruno
Publication year - 2011
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2010.100179
Subject(s) - platelet lysate , growth factor , fetal bovine serum , matrix metalloproteinase , andrology , doubling time , cell culture , cytoskeleton , cell growth , microbiology and biotechnology , in vitro , platelet , chemistry , biology , immunology , cell , medicine , biochemistry , receptor , genetics
Background: The modulation abilities of gingival fibroblasts open new therapeutic strategies for the treatment of vascular diseases (e.g., aneurism) and irradiation burns. Culture media are classically supplemented with animal sera to provide nutriments. Unfortunately, because of their potential for interspecies transmission of microorganisms, these media are not used for cells destined for human transplantation. This preliminary phenotypic study aims to test a serum‐free (SF) culture medium for human gingival fibroblasts (hGF) supplemented with human platelet lysates (PLs) for rapid cell expansion. Methods: An SF medium was first elaborated to compete with hGF proliferation in a reference medium containing 10% fetal bovine serum (BSmedium). Adhesion, proliferation, and doubling kinetics were run in the presence of PLs (SF+PL). Cytoskeletal proteins were analyzed and chromosomal abnormalities were evaluated by karyotype analyses. The SF+PL influence on secretion of molecules implied in tissue remodeling (i.e., matrix metalloproteinases [MMPs], their tissue inhibitors [TIMPs], and several growth factors) was studied. Results: SF+PL increased the proliferation rate 1.5‐fold in a week compared to BSmedium. Cytoskeleton protein expression was similar in BSmedium and in SF+PL. Chromosomal abnormalities were rare in SF+PL. MMP‐1, MMP‐2, MMP‐3, MMP‐7, MMP‐9, TIMP‐1, and the growth factors interleukin‐1β and ‐4 and transforming growth factor‐β1 secretions were stable during the experiment. TIMP‐2 and interleukin‐6 were slightly decreased in SF+PL compared to BSmedium. Conclusion: While waiting confirmation from a proteomic approach, this SF culture medium could allow a secured faster hGF proliferation adapted for human cell transplant therapy.