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Immunologic and Microbiologic Profiles of Chronic and Aggressive Periodontitis Subjects
Author(s) -
Rescala Bruno,
Rosalem Wilson,
Teles Ricardo P.,
Fischer Ricardo G.,
Haffajee Anne D.,
Socransky Sigmund S.,
Gustafsson Anders,
Figueredo Carlos Marcelo
Publication year - 2010
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2010.090643
Subject(s) - aggressive periodontitis , gingivitis , chronic periodontitis , medicine , periodontitis , dentistry , immunology , gastroenterology
Background: This study determines the gingival crevicular fluid (GCF) levels of interleukin (IL)‐1β, IL‐2, IL‐4, IL‐8, interferon (IFN)‐γ and elastase activity in inflamed shallow and deep periodontal sites from patients with generalized chronic (GCP) and generalized aggressive periodontitis (GAgP), and to compare them to shallow sites from subjects with gingivitis. A secondary aim analyzes the microbiologic profile of these subjects. Methods: Cross‐sectional clinical data were obtained from 20 GCP, 17 GAgP, and 10 gingivitis subjects. GCF samples were collected with paper strips and the levels of IL‐1β, IL‐2, IL‐4, IL‐8, and IFN‐γ were measured using a multiplexed bead immunoassay. Elastase activity was assessed by an enzymatic assay. Subgingival plaque samples were analyzed using checkerboard DNA‐DNA hybridization. Significance of differences among groups for immunologic and microbiologic data was examined using Kruskal‐Wallis adjusting for multiple comparisons. Results: Mean clinical parameters and GCF volumes were higher in patients with GCP and GAgP compared to the gingivitis group. Higher levels of IL‐1β and higher elastase activity were found in deep sites compared to shallow sites in both periodontitis groups ( P <0.05). The microbiologic data showed significantly higher levels of the red complex species in patients with GCP and GAgP compared to gingivitis ( P <0.05). There were no statistically significant differences in levels of GCF biomarkers and in levels of subgingival bacterial species between subjects with GCP and GAgP. Conclusion: There were no statistically significant differences in the measured immunologic and microbiologic parameters between subjects with GCP and GAgP.