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Grape Seed Extract Suppresses Lipopolysaccharide‐Induced Matrix Metalloproteinase (MMP) Secretion by Macrophages and Inhibits Human MMP‐1 and −9 Activities
Author(s) -
La Vu Dang,
Bergeron Chantal,
Gafner Stefan,
Grenier Daniel
Publication year - 2009
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2009.090251
Subject(s) - matrix metalloproteinase , secretion , lipopolysaccharide , aggregatibacter actinomycetemcomitans , grape seed extract , periodontal pathogen , recombinant dna , chemistry , periodontitis , microbiology and biotechnology , biology , biochemistry , porphyromonas gingivalis , immunology , medicine , pathology , alternative medicine , gene
Background: Matrix metalloproteinases (MMPs) produced by resident and inflammatory cells in response to Gram‐negative periodontopathogens play a major role in the tissue destruction observed during periodontitis, a disease that affects tooth‐supporting structures. In this study, we investigated the effect of grape seed extract (GSE) on MMP secretion by human monocyte‐derived macrophages stimulated with Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans ) lipopolysaccharide (LPS) and on the activity of human recombinant MMP‐1 and −9. Methods: Macrophages were treated with various concentrations of GSE prior to being stimulated with A. actinomycetemcomitans LPS. The secretion of MMPs and activation of nuclear factor‐kappa B (NF‐κB) p65 and activator protein‐1 (AP‐1) were assessed by enzyme‐linked immunosorbent assay (ELISA). The effect of GSE on the catalytic activity of human recombinant MMP‐1 and −9 was tested using fluorogenic assays. Results: GSE inhibited the secretion of MMP‐1, −3, −7, −8, −9, and −13 by LPS‐stimulated macrophages in a concentration‐dependent manner. The suppression of MMP secretion was associated with inhibition of NF‐κB p65 and AP‐1 activation. Also, GSE dose‐dependently inhibited the activity of MMP‐1 and −9. Conclusion: The present study suggests that GSE may be potentially used in the development of novel host‐modulating strategies for the treatment of MMP‐mediated disorders such as periodontitis.